After the researchers induced myocardial infarction in the mice during their experiments, the extra stores of p27 in the caffeinated cells apparently prevented damaged heart muscle cells from dying. The paper says the mitochondrial p27 also triggered the creation of cells armed with strong fibers to withstand mechanical forces, and promoted repairs to the linings of blood vessels and the inner chambers of the heart. To confirm the protein’s importance, the scientists engineered mice with a p27 deficiency. Those mice were found to have impaired mitochondrial function that did not improve with caffeine.
Coffee is 99% water. While this may seem obvious, we often don’t take into account the quality of that water when brewing a morning cup. Start with the highest quality ingredients, and you will ensure that best coffee possible. This goes for the beans, too. Always opt for organic, and spend the extra dollars if you have to. Coffee is the most heavily sprayed crop in the world, pesticide-wise, so you really don’t want to go with beans of dubious quality.
If you're getting your coffee at a coffee shop, make sure to avoid all of those fancy specialty coffees (sweetened flavored lattes, frappuccinos, etc) as they are almost ALWAYS loaded with extra sugars or artificial sweeteners.  Some of those fancy coffee drinks at Starbucks or other coffee shops can have 300-400 calories in just one coffee!  Definitely not good for your body or your blood sugar or insulin levels. 
What I do instead is use either a very small touch of organic maple syrup or a half packet of natural stevia to just lightly sweeten my coffee.  I've also become a big fan of coconut sugar recently, and this is healthier than plain sugar because it does contain some minerals and other nutrients, and has a lower glycemic index than regular sugar.   On the other hand, if you like your coffee black with no sweetener at all, that's the healthiest way.
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In fact, coffee provides the biggest source of antioxidants for most Americans... although that mostly has to do with the fact that many Americans don't get enough antioxidants from fruits and veggies, so coffee ends up being their biggest source.  You should try to diversify your sources of antioxidants from fruits, veggies, spices, herbs, berries, beans, unsweetened organic cocoa, teas, and yes, even coffee if you like it.

Bulletproof coffee is considered a “healthier” version of coffee because it has butter and coconut oil, which contains medium-chain fats that have been shown in studies to have a beneficial effect on blood lipids—lowering triglycerides and raising HDLs. As president and chief culinary officer at Culinary Health Solutions, Ken Immer, CCHE explains, “The fats from the butter and coconut milk are a great combination to ‘prime the energy pump’ in the morning. You’ll give your coffee some ‘bite’ that keeps you going until lunchtime, plus you’ll get all of the additional nutrients, especially when we choose grass-fed butter.” You can either buy Bulletproof or make it yourself at home. Find out 8 myths about coffee you should know.
I also tried his Bulletproof Coffee which is much smoother than regular coffee and which he claims is produced carefully to avoid the presence of mycotoxins: “Most coffee beans are processed by either leaving them in the sun and elements to wither and dry, or by pressing them and letting them ferment (spoil) to remove the outer layer of the bean. Both of these techniques are known to produce significant levels of mycotoxins as they enhance flavor. Upgraded coffee beans are mechanically processed right after picking using only clean cold water. This more expensive process is safer because it dramatically reduces harmful molds or bacteria from impacting your health.”
After one too many long nights at the office, it’s not uncommon to experience mental fatigue. In addition to getting your mental health back on track to make sure it doesn’t lead to more serious health problems, drink some coffee: A 2010 review in the journal Nutrition found caffeine can help decrease the exhaustion you’re feeling by perking your body up.
In 2005, Hu's team reviewed nine studies on coffee and type 2 diabetes. Of more than 193,000 people, those who said they drank more than six or seven cups daily were 35% less likely to have type 2 diabetes than people who drank fewer than two cups daily. There was a smaller perk -- a 28% lower risk -- for people who drank 4-6 cups a day. The findings held regardless of sex, weight, or geographic location (U.S. or Europe).

For a long time, research showed drinking coffee was associated with a higher risk of conditions like heart disease. Later, scientists identified a problem with many of the studies: they didn’t control for other behaviors. Coffee drinkers, it turns out, are also more likely to smoke, drink, and be inactive, all behaviors that raise the risk of many chronic diseases.
Historically, it was first recorded as a drink just over 500 years ago, beginning on the Arabian peninsula, but there is a speculation that its use as a stimulating beverage stretches back more than 1,000 years in various ancient and indigenous cultures. Now, it is consumed in nearly every country of the world, and almost daily, even 5-6 times a day. While offices have larger machines, at home people often have single serve coffee makers which work best for the small requirements.

Don’t feel bad about those days you drink a little too much coffee: A 2016 study published in the Journal of Neurology, Neurosurgery, and Psychiatry found drinking a high consumption—we’re talking more than four a day—can help reduce your risk of multiple sclerosis, a disease that causes the immune system to attack the protective covering of the nerves in the brain, spine, and eyes. And not just a little—by 31 percent.
Malignant melanomas are the most dangerous and potentially life-threatening form of skin cancer and one of the most common cancer types in the United States. Studies have found that there is potentially as much as a 20 percent lower risk when a person drinks a minimum of four cups of coffee daily. The type of coffee matters here, as the study showed that decaffeinated coffee was not as effective. Studies on non-melanoma cancer and coffee have shown that people were 17 percent less likely to develop the most common form of skin cancer, basal cell carcinoma, if they drank three or more cups of coffee daily.
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